Sondy TaqMan

Dual-labeled probes TaqMan

TaqMan probes are oligonucleotides with a reporter fluorophore, a quencher and a 3' extension block.

During the PCR reaction these probes will be sequence-specifically hydrolysed, thus releasing the fluorophore (5' nuclease assay)

TaqMan probes like Molecular Beacons and FRET Probes are used for Real-Time detection of PCR products.

All products undergo a multi-step HPLC purification to ensure complete labeling; unquenched molecules increase the intrinsic fluorescence (higher background)

FastProbes with 6-FAM reporter fluorophore and 3'-Dabcyl or 3'-TAMRA quencher are synthesized and fully labeled in one step followed by multi-step purification procedure.

'Classic TaqMan' (FAM/TAMRA) labeled at its5' with fluorescein and internal dT-TAMRA or 3' terminal dT-TAMRA. The 3'-end is protected against polymerase extension by phospohorylation.

DuoProbes offer  a higher flexibility allowing you to chose from a variety of reporter fluorophores (amidite chemistry) and different quenchers.

DuoPlus increases the spectrum of available reporter fluorophores. Please allow longer turnaraound times due to the more complex production process.

We design your Real-Time PCR assay free of charge.

FastProbes with 6-FAM reporter fluorophore and 3'-Dabcyl or 3'-TAMRA quencher are synthesized and fully labeled in one step followed by multi-step purification procedure.
The quencher TAMRA (rhodamin) is by itself a fluorophore, absorved energy is emitted as fluorescence. 'Dark Quencher' do not emit light and therefore, do not interfere with the measurement. 'Dark Quencher' Dabcyl [DiAzoBenzyl, = Methylorange].

Classic TaqMan probe (FAM-int.dT-TAMRA, 3'-phosphate)
Nazwa	Ilość dostarczona	Ilość reakcji
Classic TaqMan for the EVAluation , 6FAM reporter and Dabcyl or TAMRA as quencher.	300 pmol	100
Classic TaqMan Probe	1.0 OD	1 000
Classic TaqMan Probe	2.0 OD	2 000
Classic TaqMan Probe	5.0 OD	5 000
Classic TaqMan Probe	10 OD	10 000
Classic TaqMan Probe	30 OD	30 000
Additional nucleotides for probes longer than 30 bases

 'Classic TaqMan' (FAM/TAMRA) labeled at its5' with fluorescein and internal dT-TAMRA or 3' terminal dT-TAMRA. The 3'-end is protected against polymerase extension by phospohorylation.

DuoProbe (TaqMan probe)
Nazwa	Ilość dostarczona	Ilość reakcji
Duo TaqMan for the EVAluation	300 pmol	100
Duo TaqMan Probe	1.0 OD	1 000
Duo TaqMan Probe	2.0 OD	2 000
Duo TaqMan Probe	5.0 OD	5 000
Duo TaqMan Probe	10 OD	10 000
Duo TaqMan Probe	30 OD	30 000
Additional nucleotides for probes longer than 30 bases

Duo TaqMan probes with 5'-Reporter (FAM, TET, HEX, YAK, Cy3, Cy5) and terminal quencher (Dabcyl, TAMRA, BHQ1™, BHQ2™).
The quencher TAMRA (rhodamin) is by itself a fluorophore, absorved  energy is emitted as fluorescence. 'Dark Quencher' do not emit light and therefore, do not interfere with the measurement. 'Dark Quencher' Dabcyl [DiAzoBenzyl, = Methylorange].
Dabcyl and BHQ1™ can be used for dyes with an emmission wave length of up to 600 nm. BHQ1™ and BHQ2™ cover wave lenghts up to 700 nm.

DuoProbePlus (TaqMan probe)
Nazwa	Ilość dostarczona	Ilość reakcji
DuoPlus TaqMan for the EVAluation	300 pmol	100
DuoPlus TaqMan Probe	1.0 OD	1 000
DuoPlus TaqMan Probe	2.0 OD	2 000
DuoPlus TaqMan Probe	5.0 OD	5 000
DuoPlus TaqMan Probe	10 OD	10 000
DuoPlus TaqMan Probe	30 OD	30 000
Additional nucleotides for probes longer than 30 bases

DuoProbe Plus TaqMan pobes with 5'-reporter (JOE, TAMRA, ROX, TEXAS, Bodipy, MegaStokes) and terminal quencher (Dabcyl, TAMRA, BHQ1™, BHQ2™).